Emil Pai

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Projets de recherche récents

How enzymes break carbon-fluorine bonds

2015/16-2019/20 • $190,000 • PI
Canada has identified and classified over 22,000 contaminated or suspected contaminated sites. Many of them harbour halogenated hydrocarbons, contaminants that are highly resistant to natural breakdown as well as remediation efforts. Some of these compounds can even be found in the blood of...

Upgrade Advanced EPR/DEER Spectroscopy Facility

2019/20 • $150,000 • PI,CO
Electron paramagnetic resonance (EPR) and double electronelectron resonance (DEER) spectroscopy are important structural biology methods to obtain information on protein dynamics and protein conformations, which is complementary to cryo-electron microscopy (cryo-EM) and x-ray crystallography....

How enzymes break carbon-fluorine bonds

2010/11-2014/15 • $416,750 • PI
Dehalogenase enzymes break carbon-halogen bonds. Most remarkable are enzymes able to break the most stable bond in organic chemistry, the carbon-fluorine bond. Supported by an NSERC Strategic Grant and in collaboration with E. Edwards (U of T) and A.Yakunin (SGC), we screened microbial genomes...

Core facility infrastructure for optimizing conditions for lipidic cubic phase-based membrane protein crystallization

2014/15 • $150,000 • PI,CO
Membrane proteins perform a variety of biological functions that are critical to the survival of bacteria, plants, and eukaryotes. Crystal structures of these membrane proteins have shed enormous insights into the functions of transporters, signal transduction receptors, and enzymes. However,...

Examining Biomolecular Interactions using Microscale Thermophoresis

2013/14 • $150,000 • PI,CO
As part of a team of 10 structural biologists at the University of Toronto (spanning several departments and faculties), we use biophysical tools such as Atomic Force Microscopy, NMR spectroscopy, X- ray crystallography and electron cryomicroscopy to analyze the biomolecular interactions between...