A New Ciboria sp. for Soil Mycoremediation and the Bacterial Contribution to the Depletion of Total Petroleum Hydrocarbons

Frontiers in Microbiology, Vol. 12 (2021)

Mots clés
Auteurs
  • Simone Becarelli
  • Department of Biology, University of Pisa, Pisa, Italy
  • Simone Becarelli
  • BD Biodigressioni, Pisa, Italy
  • Ilaria Chicca
  • Department of Biology, University of Pisa, Pisa, Italy
  • Ilaria Chicca
  • Department of Biosystem Engineering, University of Manitoba, Winnipeg, MB, Canada
  • Salvatore La China
  • Department of Life Sciences, University of Modena and Reggio-Emilia, Reggio Emilia, Italy
  • Giovanna Siracusa
  • Department of Biology, University of Pisa, Pisa, Italy
  • Alessandra Bardi
  • Department of Civil and Environmental Engineering, University of Florence, Florence, Italy
  • Maria Gullo
  • Department of Life Sciences, University of Modena and Reggio-Emilia, Reggio Emilia, Italy
  • Giulio Petroni
  • Department of Biology, University of Pisa, Pisa, Italy
  • David Bernard Levin
  • BD Biodigressioni, Pisa, Italy
  • David Bernard Levin
  • Department of Biosystem Engineering, University of Manitoba, Winnipeg, MB, Canada
  • Simona Di Gregorio
  • Department of Biology, University of Pisa, Pisa, Italy

Résumé

A Ciboria sp. strain (Phylum Ascomycota) was isolated from hydrocarbon-polluted soil of an abandoned oil refinery in Italy. The strain was able to utilize diesel oil as a sole carbon source for growth. Laboratory-scale experiments were designed to evaluate the use of this fungal strain for treatment of the polluted soil. The concentration of total petroleum hydrocarbons (TPH) in the soil was 8,538 mg/kg. Mesocosms containing the contaminated soil were inoculated with the fungal strain at 1 or 7%, on a fresh weight base ratio. After 90 days of incubation, the depletion of TPH contamination was of 78% with the 1% inoculant, and 99% with the 7% inoculant. 16S rDNA and ITS metabarcoding of the bacterial and fungal communities was performed in order to evaluate the potential synergism between fungi and bacteria in the bioremediation process. The functional metagenomic prediction indicated Arthrobacter, Dietzia, Brachybacerium, Brevibacterium, Gordonia, Leucobacter, Lysobacter, and Agrobacterium spp. as generalist saprophytes, essential for the onset of hydrocarbonoclastic specialist bacterial species, identified as Streptomyces, Nocardoides, Pseudonocardia, Solirubrobacter, Parvibaculum, Rhodanobacter, Luteiomonas, Planomicrobium, and Bacillus spp., involved in the TPH depletion. The fungal metabolism accelerated the onset of specialist over generalist bacteria. The capacity of the Ciboria sp. to deplete TPH in the soil in treatment was also ascertained.

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