BIONOTE as an Innovative Biosensor for Measuring Endocannabinoid Levels

Sensors, Vol. 21 (2021)

Mots clés
Authors
  • Simone Grasso
  • Department of Science and Technology for Humans and the Environment, Campus Bio-Medico University of Rome, 00128 Rome, Italy
  • Marco Santonico
  • Department of Science and Technology for Humans and the Environment, Campus Bio-Medico University of Rome, 00128 Rome, Italy
  • Giorgio Pennazza
  • Department of Engineering, Campus Bio-Medico University of Rome, 00128 Rome, Italy
  • Alessandro Zompanti
  • Department of Engineering, Campus Bio-Medico University of Rome, 00128 Rome, Italy
  • Alessandra Piccoli
  • Department of Medicine, Campus Bio-Medico University of Rome, 00128 Rome, Italy
  • Tiziana Bisogno
  • Institute of Translational Pharmacology, National Research Council, 00133 Rome, Italy
  • Mauro Maccarrone
  • Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, Italy

Résumé

In this study, a novel approach was developed to quantify endocannabinoids (eCBs), and was based on the liquid biosensor BIONOTE. This device is composed of a probe that can be immersed in a solution, and an electronic interface that can record a current related to the oxy-reductive reactions occurring in the sample. The two most representative members of eCBs have been analysed in vitro by BIONOTE: anandamide (N-arachidonoylethanolamine, AEA) and 2-arachidonoylglycerol (2-AG). Bovine serum albumin was used to functionalize the probe and improve the sensibility of the whole analytical system. We show that BIONOTE is able to detect both AEA and 2-AG at concentrations in the low nanomolar range, and to discriminate between these eCBs and their moieties arachidonic acid, ethanolamine and glycerol. Notably, BIONOTE distinguished these five different molecules, and it was also able to quantify AEA in human plasma. Although this is just a proof-of-concept study, we suggest BIONOTE as a cheap and user-friendly prototype sensor for high throughput quantitation of eCB content in biological matrices, with an apparent diagnostic potential for tomorrow’s medicine.

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