David Juncker

Key Research Areas
Associated Researchers
Associated Organizations

Recent Research Projects

Mechanisms of Met activation in cancer and therapeutic intervention

2015/16-2019/20 • $2,981,189 • PI,Other
Challenge Current therapies are ineffective in many aggressive cancers, which are often refractory to therapeutic interventions and develop drug resistance. Combination therapies could effectively address this issue, however, to select the right combination will require a greater molecular...

Cellular mechanotransduction in the actin cytoskeleton.

2015/16-2019/20 • $565,661 • PI,Other
Program Direction and Overall vision:Mechanics are at the heart of cell biology, and disorders are the basis of many diseasesForce-sensing and mechanical adaptation can be seen in nearly every aspect of our physiology, and cell function appears to be tied to the mechanical state of the cell. We...

Additive manufacturing of organs-on-a-chip using biodegradable elastomeric polymers

2017/18-2019/20 • $545,244 • PI,CO
Organ-on-a-chip engineering could revolutionize new compound screening and biomarker discovery. However, current platforms only reproduce a very limited set of organs and lack many critical organ functions such as vasculature, which greatly limits their potential. Recently, we developed...

Elements and systems for ultrasensitive protein analysis

2016/17-2019/20 • $432,000 • PI
The single most significant factor determining the outcome of many diseases, and notably cancer, is disease progression at diagnosis. Unfortunately, diseases are typically only detected at an advanced stage, after symptoms appear. A hallmark of diseases is the dysregulation of proteins in cells,...

Integrative and Translational Biomedical Engineering

2018/19-2019/20 • $300,000 • PI
Multiomic analysis is empowering precision medicine and is leading to a new paradigm of preventative and prognostic medicine integrating omic and physiological data. Extracellular vesicles and (rare) circulating cells carry the molecular fingerprints of their origin, and provide context-rich...

Microfiltration of Circulating and Disseminated Tumor Cells in Ovarian Cancer: From Monitoring Therapeutic Response Towards Predicting Disease Outcome

2018/19-2019/20 • $201,874 • PI,CO
Circulating and disseminated tumor cells (CTCs, DTCs) that dissociate from the primary tumor, can be found, respectively, in blood and ascites of ovarian cancer patients, and could be used to monitor the response to therapy, and help track the tumor state. CTCs and DTCs are very rare and present...

Aptamer-based enrichment system and capillary chip for low-abundance water contaminant detection to ensure aquaculture safety and quality

2016/17-2018/19 • $552,000 • PI,CO
Aquaculture is the farming of fish, shellfish and aquatic plants in fresh or seawater and has been a rapidly growing economy output of $733 millions in Canada. Contaminants with pharmaceutical effects are seeping from the sewage into natural water systems, and marine toxins persist in surface...

Bio-integrated fully implantable peripheral nerve electrode for functional upper limb prostheses

2016/17-2018/19 • $247,080 • PI,CO
The development of electrodes that can be implanted into nerves and used to communicate with robotic limbs is an exciting advance for patients with upper limb amputations. In two recent experimental cases, electrodes were implanted into human nerves, enabling the recipients to remotely control a...

Rapid diagnostic chips for detection and determination of the severity of urinary tract infection

2017/18-2018/19 • $180,000 • PI
Children presenting a fever without known sources need to be checked for bacterial urinary tract infection (UTI), which may involve infection in the kidneys, or acute pyelonephritis (APN), that can result in permanent kidney damage. Current tests involve costly and lengthy urine culturing for...

Colocalization-by-linkage assay on microparticles (CLAMP): A multiplex immunoassay platform for quantitative proteomics

2018/19 • $125,000 • PI
Proteins are the most commonly used biomarker analyzed in the clinic, and they are also measured for research and drug development. Today, the global immunoassay market for protein analysis is valued at $20B. For the past 50 years, the gold standard protein detection method has remained the...

Zetasizer Ultra for Advanced Characterization of Microplastics, Nanomaterials and Biocolloids

2018/19 • $121,145 • PI,CO
We are requesting a Zetasizer Ultra with the capability to measure size, surface charge and concentration of a wide range of micro- and nano-particles (e.g., metals, polymers, bacteria, viruses, proteins) in many different types of media (e.g., seawaters, freshwaters, soil porewaters, buffers,...

Development of novel devices for assessing drugs promoting neuronal regeneration

2018/19 • $25,000 • PI
Ananda Devices is a Montreal based SME dedicated to the development and manufacturing of*microfluidic/organ-on-a-chip devices to increase reproducibility and efficiency of cellular assays. Ananda*Devices' proprietary Neuro-MFD offers a more efficient model to test drugs targeting...

Generating innovation through the use of common data: Improving the diagnosis and treatment of pediatric and adolescent mild traumatic brain injury in Canada.

2012/13-2017/18 • $766,895 • PI,CO,Other
The incidence of pediatric traumatic brain injury (TBI) in North America is alarmingly high; studies suggest that mild TBI (mTBI) make up 70-80% of all cases reported. Of particular concern is that not only do many post-concussive symptoms, which manifest as cognitive, physical and behavioural...

Bioengineering Facility for UV Structured Substrate Patterning

2017/18 • $150,000 • PI,CO
The crawling movement and force generation of biological cells is an essential part of animal life, and can be seen in diverse situations from neurons wiring together the nervous system, leukocytes fighting infection, or in disease as in the case of cancerous cells metastasizing and invading new...

NSERC CREATE Training Program in Neuroengineering

2011/12-2016/17 • $1,500,000 • PI,CO
Injury to the central nervous system (CNS), whether sustained by trauma or disease, is extraordinarily difficult to repair. Traumatic CNS and PNS (Peripheral Nervous System) injury alone affects more than 250,000 Canadians per year, often leading to lifelong disability. The goal defined by this...

High-throughput microscopy system for ultrasensitive and multiplexed nanoarray analysis

2016/17 • $148,945 • PI,CO
The single most significant factor that determines the outcome of many diseases is disease progression and diagnosis. Unfortunately, diseases are typically detected when symptoms appear when the diseases are already at an advanced stage. A hallmark of diseases is the dysregulation of proteins in...

CIHR Training Program in Integrative Approaches to Human Health

2010/11-2015/16 • $1,607,145 • PI,CO
High-throughput (eg microarrays, sequencing) and high-content (eg single cell imaging, chemical screening) assays are now an integral part of biomedicine, and have produced successes that are now finding clinical utility. This is true for a range of human diseases including cancer, diabetes,...

NSERC CREATE Training Program in Integrated Sensor Systems

2010/11-2015/16 • $1,540,500 • PI,CO
Sensors are the means by which we learn about the world. Sensors are everywhere. They are integrated into cell phones and car airbags (accelerometers that can measure movements or sudden deceleration), and engines (temperature and pressure sensors in cars and aircraft). Sensors which can measure...

Micro and Nanobioengineering

2011/12-2015/16 • $500,000 • PI
No summary

Multidimensional separation isolation and characterization of circulating tumour cells

2013/14-2015/16 • $343,124 • PI,Other
BACKGROUND. If cancer remains localized, it can often be treated, but if it metastasis, it vecome very difficult. Metastasis is thought to occur by so-called circulating tumor cells, or CTCs. These CTCs are emerging as a new element for prognosis of cancer, as well as for measuring the response...

New approaches to multiplexed protein profiling of complex samples with ultrahigh sensitivity

2011/12-2015/16 • $225,000 • PI
The diagnosis of diseases relies on advanced technologies for detecting minute amounts of antigens in the blood. However, current analytical techniques are not sufficiently sensitive to use them for early disease detection in the case of cancer and other complex diseases. Here, we propose to...

Blood-based protein test for early detection of breast cancer using antibody colocalization microarrays

2012/13-2014/15 • $210,140 • PI,CO
One in eight women will be diagnosed with cancer during her lifetime, and the earlier the cancer is detected the better the chances of survival. We propose to develop a new technology that can detect many different proteins associated with cancer in the blood of patients. The technology will be...

Multidimensional separation isolation and characterization of circulating tumour cells

2013/14-2014/15 • $209,126 • PI,CO
BACKGROUND. If cancer remains localized, it can often be treated, but if it metastasis, it vecome very difficult. Metastasis isthought to occur by so-called circulating tumor cells, or CTCs. These CTCs are emerging as a new element for prognosis ofcancer, as well as for measuring the response to...

Visualizing and Directing Dynamic DNA Nanostructure Assembly With Single-Molecule Resolution

2014/15 • $150,000 • PI,CO
The use of DNA in nanotechnology has become increasingly widespread with far-reaching advantages: these natural nanoscale building blocks are self-assembling, are able to organize species such as drugs, nanoparticles, and other biomolecules, and can store and read information to target specific...

Rapid detection of MRSA and identification of strain using an autonomous microfluidic chip with bacteriophages

2012/13-2013/14 • $343,000 • PI,CO
BACKGROUND: Bacterial infections and in particular nosocomial - hospital-acquired - infections, are agrowing problem in worldwide, especially from bacteria resistant to antibiotics, so called "superbugs". A recentstudy identified that more than 51% of intensive care unit patients are infected,...

Reorganization of Brain Microcircuits

2010/11-2011/12 • $612,500 • PI,CO
Damage to the nervous system from trauma, stroke or disease places a terrible psychological, social and financial burden on patients and their families so scientists are trying to find ways to protect and repair the nervous system. The adult nervous system has a very limited capacity for long...

Rapid detection of MRSA and identification of strain using an autonomous microfluidic chip with bacteriophages

2011/12 • $173,500 • PI,CO
BACKGROUND: Bacterial infections and in particular nosocomial - hospital-acquired - infections, are a growing problem in worldwide, especially from bacteria resistant to antibiotics, so called "superbugs". A recent study identified that more than 51% of intensive care unit patients are infected,...

Snap Chip: portable system for miniaturized, multiplexed immunoassays

2011/12 • $122,000 • PI
Immunoassays are widely used in research and in diagnostics to quantify proteins in various samples. As our understanding of biology and disease at the molecular level progresses, there is a growing demand for multiplexed molecular tests that can measure multiple parameters at once. Whereas for...

Clinical validation of an innovative multiplex antibody microarray platform

2010/11 • $150,000 • PI
Early diagnosis of cancer, and many other diseases, significantly increases chance of survival. However, simple-to-perform blood based tests are largely missing. For breast cancer for example screening, diagnosis, staging, or routine surveillance cannot be performed as a blood test. There are...

Canada Research Chair in Micro and Nanobioengineering

2010/11 • $100,000 • PI
No summary

Microfluidic detection system for profiling multiple cancer biomerkers with ultrhigh sensivity

2010/11 • $92,400 • PI,CO
In recent years, Canada has invested over a hundred million dollars on proteomics research, and one significant outcome has been the discovery of a number of candidate cancer biomarkers. It has also become clear that early diagnosis of cancer will rely on the detection of not one, but a panel of...

Microfluidic detection system for profiling multiple cancer biomarkers in serum with ultrahigh sensitivity and specificity

2010/11 • $92,400 • PI,CO
In recent years, Canada has invested over a hundred million dollars on proteomics research, and one significant outcome has been the discovery of a number of candidate cancer biomarkers. It has also become clear that early diagnosis of cancer will rely on the detection of not one, but a panel of...

Convergent development and use of a new antibody microarray platform for establishing a serum-based protein biomarker panel for breast cancer

2010/11 • $36,329 • PI,CO
Proteins are the messengers, regulators and effectors of each cell and of the human body as a whole. In diseases such as cancer, the concentration, distribution and post-translational modification of proteins are altered, and proteins therefore carry the signature of the disease. Blood is easily...

Patents

ELECTRICAL MICROVALVE AND METHOD OF MANUFACTURING THEREOF

The present invention relates to a microvalve for controlling a fluid flow in a microchannel, to a microfluidic circuit using the microvalve, and to a manufacturing method thereof. The microvalve has a first electrode located on a portion of the microchannel, a second electrode over the microchannel and substantially aligned with the first electrode forming a membrane with substantially no resilience. In function, upon application of an electric force on the first and second electrodes, the...
Related applications: WO2007CA01997, EP20070816146, CA20072703801

MICROFLUIDIC MICROARRAY SYSTEM AND METHOD FOR THE MULTIPLEXED ANALYSIS OF BIOMOLECULES

A microfluidic system for fluid transfer to a microarray includes a liquid transfer needle having a fluid conduit therein within which is defined a withholding pressure P1, and a microcompartment defined within the microarray, the microcompartment being configured to generate a capillary pressure P2 therein. The capillary pressure P2 is less than the withholding pressure P1, such that a defined amount of liquid is transferred from the liquid transfer needle into the microcompartment when the...
Related applications: EP20090700691, WO2009CA00008

METHOD AND SYSTEM FOR PRE-PROGRAMMED SELF-POWER MICROFLUIDIC CIRCUITS

A major challenge for the general use of "lab-on-a-chip" (LOAC) systems and point- of-care (POC) devices has been the generally complex and need for sophisticated peripheral equipment, such that it is more difficult than anticipated to implement low cost, robust and portable LOAC / POC solutions. It would be beneficial for chemical, medical, healthcare, and environmental applications to provide designs for inexpensive LOAC / POC solutions compatible with miniaturization and mass production,...
Related applications: EP20120826963, WO2012CA00805

METHODS AND DEVICES FOR MULTI-DIMENSIONAL SEPARATION, ISOLATION AND CHARACTERIZATION OF CIRCULATING TUMOUR CELLS

In most cancers, a significant factor in a poor outcome for the individual cancer victim is metastatic disease, i.e., dissemination of tumour cells to other parts of the human body via the circulation, such as distant organs, and their subsequent proliferation therein to form multiple other cancer tumours. The presence of circulating tumour cells, or CTCs, represents a vital intermediate step in this process and variations of a few CTCs within blood samples containing tens of billions of...
Related applications: EP20130768298, WO2013CA00272

FLUIDIC CIRCUITS AND METHODS FOR BACTERIAL SCREENING

A microfluidic device and method for performing an assay for detecting bacteria in a fluid sample are described. The microfluidic device includes a capillaric circuit having a main channel and a sample reservoir which are fluidicly connected and separated from each other by a sample-release valve integrally formed with the capillaric circuit. The main channel has a suspension of functionalized microbeads therein, which form a microbead column portion receiving the plurality of functionalized...
Related applications: WO2016CA51232

DOMINO CAPILLARY MICROFLUIDIC CIRCUIT

There is disclosed a capillary microfluidic circuit including a main channel communicating with a flow inducing element. The main channel has intermediary inlets. Reservoirs for containing one or more liquids prior to being drawn into the main channel. The reservoirs include a first reservoir and at least a second reservoir. Each of the reservoirs has an upstream end connectable to vents for filling the reservoirs with the one or more liquids and a downstream end. The downstream end of each...
Related applications: WO2018CA51322

COLOCALIZATION-BY-LINKAGE SANDWICH ASSAYS

There are provided methods and systems for detecting and/or quantifying an analyte. In particular, there are provided methods and systems for simultaneous detection and/or quantitation of two or more analytes in a sample. In some embodiments, there are provided colocalization-by-linkage assays on microparticles (CLAMP) comprising two sets of binders pre-assembled on a support, such that the two sets of binders are colocalized before contacting the sample.
Related applications: WO2019CA50405