John Brennan

Key Research Areas
Associated Researchers
Associated Organizations

Recent Research Projects

Bioanalytical Chemistry and Biointerfaces

2011/12-2018/19 • $1,400,000 • PI
The development of new materials which can interact with biological systems in a desired way is vital to many established and emerging industrial sectors, including diagnostics, drug discovery and implantable devices. Dr. Brennan will use infrastructure within the newly established Biointerfaces...

DNAPrint: a pathogen-tracking paper sensor platform that integrates functional DNA with nanomaterials

2016/17-2018/19 • $672,000 • PI,CO
Many human diseases are linked to infections involving various pathogens. In addition, growing risks of bioterrorism pose on-going threats to public safety and security. Moreover, rapid evolution of antibiotic-resistant "superbugs" is becoming a pressing global concern. It has been recognized...

Point-of-Care Diagnostics

2018/19 • $100,000 • PI
My research program will focus on the discovery, development and translation of new point-of-care assays and diagnostic devices using high-throughput approaches and common platform chemistries to allow for more rapid discovery, optimization, translation and commercialization of transformative...

Functional DNA Amplicons

2018/19 • $64,000 • PI
The long-term objectives of my research program are to further develop additional tools in the repertoire of methods for biomolecule immobilization. Primarily, we aim to investigate the nature of DNA and RNA superstructures formed from rolling circle amplification of functional nucleic acids...

High Throughput Microarray Printing and Imaging Methods to Develop new Bioactive Materials for Sensing and Small Molecule Screening

2013/14-2017/18 • $345,000 • PI
My NSERC-supported research program will develop and utilize high throughput, microarray and microwell plate-based methods to synthesize and characterize advanced sol-gel based bioactive materials that will transform solid-phase assays used in diagnostics and drug discovery. These new materials...

NSERC CREATE Biointerfaces Training Program

2011/12-2016/17 • $1,300,000 • PI,CO
Our understanding of the nature of the biological/material interface, or biointerface, impacts all materials in biological media from contact lenses to blood contacting materials to diagnostic test strips and new bioseparation media. The CREATE Biointerfaces Training Program will provide unique...

NSERC - SENTINEL - Bioactive Paper Strategic Network

2010/11-2015/16 • $7,594,000 • PI,CO
Bioactive paper that can detect and deactivate dangerous bacteria and viruses in food and water will deliver health benefits around the world. Bioactive Paper is a made-in-Canada concept articulated in the first phase (2005-2010) of the Sentinel Bioactive Paper Network, an NSERC strategic...

NSC - Design and screening of DNA-functionalized hydrogels for contaminants in water

2013/14-2015/16 • $553,600 • PI,CO
With the rapid increase in global population and continuous release of new chemical contaminants into the environment, maintaining safe and sufficient natural resources, especially water, has become increasingly challenging. It is strategically important to develop new technologies that target...

Development of diagnostic teststrips incorporating concatameric aptamers for quantitative detection of C. difficile

2013/14-2015/16 • $314,000 • PI,CO
The emergence of new pathogenic species and strains that are resistant to traditional treatments are amongst the most pressing health concerns of our time. The bird flu virus epidemic and the outbreak of Clostridium difficile in several hospitals across Canada in the past year are the latest...

The development of an eosinophil-specific bioactive paper detection system

2013/14-2014/15 • $300,000 • PI,CO
Asthma and COPD are common airway diseases, Flare-ups (exacerbations) cause significant morbidity. The majority ofthese are associated with an allergic type of bronchitis that is characterized by the presence of a white blood cell, eosinophil,that can be detected in sputum. However, this is not...

Integrating imaging surface plasmon resonance and imaging MALDI MS for protein/surface interaction studies

2013/14 • $23,100 • PI
Two important methods for imaging protein interactions at or with surfaces are imaging surface plasmonresonance (iSPR) and imaging MALDI MS/MS (iMALDI). These two methods are uniquely complementary.iSPR has the ability to measure reactions at surfaces in real-time, giving useful data on the...

Solid-phase assays for biosensing and high throughput screening using sol-gel derived bioglasses

2010/11-2012/13 • $198,000 • PI
The major objectives of the proposed research are: (1) the development of biocompatible sugar or polyol modified sol-gel precursors and new peptide functionalized bis-silanes that will provide tunable surface properties and controlled morphologies in silica materials along with good long-term...

The CREATE Molecular Imaging Probes Program

2010/11-2011/12 • $450,000 • PI,CO
The impact that was felt by the recent shortage of medical isotopes highlighted the importance that molecular imaging and the associated probes has on the health of Canadians and on the economy of Canada. It also demonstrated that there is a need to train a new generation of scientists to...

Canada Research Chair in Bioanalytical Chemistry

2010/11-2011/12 • $150,000 • PI
No summary

Validation and scale up to bioactive paper test strips for organophosphate pesticides

2011/12 • $122,000 • PI
My research group recently developed a simple, paper-based colorimetric test strip for organophosphate pesticides that has the potential to revolutionize food and water testing in resource limited settings. The test strip utilizes sol-gel based bio-immobilization methods in conjunction with...

Functional DNA based bioassays and biosensors for the detection of pathogenic bacteria in the health sector and food industry

2010/11 • $45,668 • PI,CO
The threat of bioterrorism and the emergence of new pathogenic species and strains that are resistant to traditional treatments are amongst the most pressing health concerns of our time. The bird flu virus epidemic and the outbreak of Escherichia coli and Salmonella-contaminated lettuce in North...

Patents

METHODS AND COMPOUNDS FOR FORMING MONOLITHIC TITANIA, OPTIONALLY BIOMOLECULE DOPED, WITH CONTROLLED MORPHOLOGY USING BIOCOMPATIBLE SOL-GEL PROCESSES

The present invention includes polyol modified titanium compounds, their preparation and use in methods to make biomolecule compatible monolithic tit ania. The invention also includes the use of the biomolecule compatible mono lithic titania in bioanalytic applications, for example in biosensors, chrom atographic columns, microarrays and bioaffinity columns.
Related applications: WO2007CA01693, CA20072664218

BIOSENSORS UTILIZING INK JET-PRINTED BIOMOLECULE COMPATIBLE SOL GEL INKS AND USES THEREOF

Novel solid-phase biosensors that utilize ink jet printing of biocompatible sol-gel based inks to create sensor strips are reported herein. Biomolecules and other reagents useful in bioassays to detect, for example, pathogenic microorganisms or toxic substances, are immobilized on a substrate, which can be paper based, by layering these substances between two layers of biomolecule compatible sol gel. The sol gel precursor solutions and solutions of the assay reagents are printed from...
Related applications: EP20100779977, CA20102763834, WO2010CA00802

BIOSENSORS COMPRISING CONCATEMERIC NUCLEIC ACID MOLECULES

The present application relates to biosensors, for example comprising an high-molecular weight, tandem repeating, structure-switching nucleic acid aptamers (concatemeric aptamers) to rapidly create patterned sensors via, for example, inkjet printing. These concatemeric aptamer reporters remain immobilized at the point of printing through strong adsorption but retain sufficient segmental mobility to undergo structure switching and reporter signalling to provide both qualitative and...
Related applications: CA20162925053

BIOSENSOR COMPRISING TANDEM REACTIONS OF STRUCTURE SWITCHING, NUCLEOLYTIC DIGESTION AND AMPLIFICATION OF A NUCLEIC ACID ASSEMBLY

The present application relates to a biosensor for detecting analytes, various kits and methods of use thereof. In particular, the biosensor's mode of operation is based on binding of analytes to a nucleic acid sequence which triggers rolling circle amplification and detection of the amplified product as the indicator of the presence of the analytes.
Related applications: EP20160813435, WO2016CA50731, CA20162989445

NUCLEIC ACID CATENANE WITH A LINKING DUPLEX BIOSENSOR FOR DETECTION OF A MICROORGANISM TARGET

The present application is directed to biosensors and methods for detecting a microorganism target in a sample using a mechanically interlocked nucleic acid catanane, wherein an enzyme from the microorganism target or that is activated by a molecule from the microorganism target cleaves a linkage in a first single-stranded nucleic acid ring of the catanane structure, allowing rolling-circle amplification to occur and the presence of rolling-circle amplification products indicates the...
Related applications: CA20163046266, WO2016CA51459

NUCLEIC ACID AMPLIFICATION BIOSENSOR FOR USE IN ROLLING CIRCLE AMPLIFICATION (RCA)

The present application describes a biosensor for detecting target nucleic acid. The biosensor's mode of operation is based on binding of the target nucleic acid to another nucleic acid sequence and a circular template which triggers rolling circle amplification and detection of the amplified product as the indicator of the presence of the target nucleic acid. The biosensor is immobilized on a solid support, such as paper.
Related applications: CA20163007848, WO2016CA51458

NUCLEIC ACID ENZYME-MEDIATED SIGNAL AMPLIFICATION FOR BIOSENSING

Described is an approach that takes advantage of rolling circle amplification (RCA) and an RNA-cleaving DNAzyme (RCD) to achieve massive signal amplification for biosensing via a cross-feedback mechanism. An RCA reaction generates copies of an RCD that triggers a reaction cascade designed to generate additional DNA assemblies for RCA. These cross- feedback actions work autonomously to turn limited molecular recognition events into massive amounts of DNA amplicons that can be conveniently...
Related applications: WO2018CA50294

SOL-GEL DERIVED BIOHYBRID MATERIALS INCORPORATING LONG-CHAIN DNA APTAMERS

The present application relates to a new class of macroporous bio/inorganic hybrids, engineered through a high-throughput materials screening approach that entrap micron-sized concatemeric DNA aptamers. The entrapment of these long-chain DNA aptamers allows their retention within the macropores of the silica material, so that aptamers can interact with high molecular weight targets such as proteins.
Related applications: CA20183006158

APTAMERS FOR CLOSTRIDIUM DIFFICILE DETECTION

Provided herein are aptamers, aptamer probes and biosensor systems that detect C. difficile glutamate dehydrogenase (GDH). Also provided are methods of detecting C. difficile GDH using the aptamers, probes and biosensors and methods of determining whether a subject has a C. difficile infection.
Related applications: CA20183016351