Paul Wiseman

Key Research Areas
Associated Researchers
Associated Organizations

Recent Research Projects

New Dimensions and Domains for Fluorescence Fluctuation Imaging Methods

2017/18-2019/20 • $318,000 • PI
Advancing quantitative image correlation methods to the third dimension and beyond In the past decade there has been a revolution in light microscopy recognized with the awarding of the 2014 Chemistry Nobel Prize to Betzig, Moerner and Hell for the development of super resolved fluorescence...

Replacing a supercontinuum ultrafast fiber laser with tunable outputs in the Visible and NIR and adding a UV source at 375 nm

2019/20 • $145,793 • PI,CO
This is an application for funds to replace a supercontinuum ultrafast fiber laser with pulsed (6ps fwhm), tunable (up to 8 simultaneous) outputs in the Visible and Near Infrared range of the spectrum, from 450 nm to 1,400 nm. The laser replaces one previously acquired with an NSERC RTI-1 award...

Super-resolution microscopy network analysis: Drug target validation for cystic fibrosis

2019/20 • $115,850 • PI,CO
The goal of genome-based precision medicine is to selectively target drugs to responsive patients. This is particularly important in cystic fibrosis (CF), where ~75% of eligible patients, carrying the F508del-CFTR mutation, do not show demonstrable improvement in lung function with the...

Sensitive and rapid sCMOS camera for fluorescence fluctuation imaging

2018/19 • $37,492 • PI,CO
This NSERC RTI grant will support the purchase of a back illuminated sCMOS camera to replace an inoperable and obsolete electron multiplying charged coupled device (EMCCD) camera for fluorescence imaging on our main lab microscope. CMOS image sensors are used in almost all cell phone cameras to...

NSERC CREATE Training Program in Bionanomachines

2011/12-2016/17 • $1,650,000 • PI,CO
No summary

NSERC CREATE Training Program in Neuroengineering

2011/12-2016/17 • $1,500,000 • PI,CO
Injury to the central nervous system (CNS), whether sustained by trauma or disease, is extraordinarily difficult to repair. Traumatic CNS and PNS (Peripheral Nervous System) injury alone affects more than 250,000 Canadians per year, often leading to lifelong disability. The goal defined by this...

Image correlation studies of distributions and dynamics of macromolecules in cells

2012/13-2016/17 • $505,000 • PI
One of the major scientific challenges in the era of emergent quantitative biology and molecular medicine will be to develop a physico-chemical understanding of how biological macromolecules interact within living cells so as to control biochemical processes. Currently, there are only a limited...

High-throughput microscopy system for ultrasensitive and multiplexed nanoarray analysis

2016/17 • $148,945 • PI,CO
The single most significant factor that determines the outcome of many diseases is disease progression and diagnosis. Unfortunately, diseases are typically detected when symptoms appear when the diseases are already at an advanced stage. A hallmark of diseases is the dysregulation of proteins in...

NSERC CREATE Training Program in Biophotonics

2010/11-2015/16 • $1,646,225 • PI,CO
The future of progress in Life Sciences, from understanding basic biochemistry to complex biological organisms and environmental issues, hinges critically on our ability to improve our ways to study and manipulate molecules and living organisms with increased precision and reduced invasiveness....

Visualizing and Directing Dynamic DNA Nanostructure Assembly With Single-Molecule Resolution

2014/15 • $150,000 • PI,CO
The use of DNA in nanotechnology has become increasingly widespread with far-reaching advantages: these natural nanoscale building blocks are self-assembling, are able to organize species such as drugs, nanoparticles, and other biomolecules, and can store and read information to target specific...

Supercontinuum ultrafast fiber laser with tunable outputs

2013/14 • $97,065 • PI,CO
This is an application for funds to acquire a supercontinuum ultrafast fiber laser with tunable (up to 8 simultaneous) outputs from 410 nm to 2,000 nm. The laser will be integrated to our combined setup for single molecule imaging (SMI), fluorescence lifetime imaging (FLIM), fluorescence...

Patents

FLUORESCENT PHOTOPOLYMERIZABLE RESINS AND USES THEREOF

There is provided fluorescent photopolymerizable resins for use in biological studies and image acquisition. In particular the photopolymerizable resins are useful in studying the properties of cells. The resins are also useful for the calibration of microscopic measurement systems.
Related applications: WO2006CA00963

HAEMOZOIN DETECTION

A system for detecting haemozoin in a sample, said system including a light source for exciting the sample with an optical signal to generate a non-linear optical response, and a detector for detecting the generated non-linear optical response from the excited sample. A method for detecting haemozoin in a sample, the method including exciting the sample with an optical signal to generate a non-linear optical response from the sample, and detecting the emitted non-linear optical signal from...
Related applications: WO2008CA01314