Mitchell Winnik


Contact via University of Toronto


Key Research Areas
Associated Researchers
Associated Organizations

Recent Research Projects

High sensitivity reagents for bioassays employing mass cytometry

2015/16-2019/20 • $598,750 • PI,CO
This proposal describes the synthesis, characterization, and biofunctionalization of inorganic nanoparticles designed as high-sensitivity reagents for mass cytometry (CyTOF), a technique invented and commercialized by our partner Fluidigm Canada in Markham ON. Mass cytometry works on the...

Polymer Conformation, Dynamics, Morphology

2017/18-2019/20 • $372,000 • PI
My research is focused on developing new fundamental scientific knowledge about polymers as materials in areas where one can have an impact on modern technology or medicine. Much of our effort is devoted to the creation and study of polymer objects that form stable colloids in solution, with a...

Monitoring diffusion and reaction rate during film formation of waterborne 2-pack polyurethane coatings

2018/19-2019/20 • $254,250 • PI
Concern for the environment has been driving major changes in technology in the coatings industry. To reduce the amount of volatile organic compounds (VOC's) released to the atmosphere, new technology is being developed to replace solvent-based coatings with water-based coatings. The biggest...

Liquid flow-cell sample holder for liquid-phase electron microscopy and correlative microscopy research

2019/20 • $135,984 • PI,CO
Scientific breakthroughs often build on the successful visualization of objects invisible to the naked eye. Electron microscopy is one of the most powerful characterization techniques for studying structures of materials and related phenomena down to atomic/molecular length scales. We request to...

Ultracentrifugation Facility

2018/19 • $103,009 • PI,CO
High-speed ultracentrifugation is essential for diverse areas of modern chemical research. This multi-purpose instrument is required by many labs in our department, particularly those working in the areas of biological and materials chemistry. For example, ultracentrifugation is essential for...

Polymer conformation, Dynamics, and Morphology

2012/13-2016/17 • $855,000 • PI
My research is focused on developing new fundamental scientific knowledge about polymers as materials in areas where one can have an impact on modern technology. Much of our effort is devoted to the creation and study of polymer objects that form stable colloids in solution, with a well-defined...

MALDI mass spectrometer

2016/17 • $150,000 • PI,CO
Matrix Assisted Laser Desorption Ionization (MALDI) mass spectrometry allows the rapid characterization of large molecules such as peptides, proteins and polymers. There is currently no MALDI mass spectrometer in the chemistry department at the University of Toronto. There are 10 research groups...

Determining nanoparticle size distribution and concentration for bioanalytical applications

2016/17 • $142,057 • PI,CO
This proposal requests funds for a Nanoparticle Tracking Analysis (NTA) instrument that enables one to measure the size, the number distribution, and number concentration of colloidal nanoparticles (NPs). While there are many applications of this type of instrument, we are particularly...

Understanding polymer diffusion in thermoset epoxy-based waterborne coatings and its influence on final film properties

2015/16-2016/17 • $99,979 • PI
Concern for the environment has been driving major changes in technology in the coatings industry. To reduce the amount of volatile organic compounds (VOC's) released to the atmosphere, new technology is being developed to replace solvent-based coatings with water-based coatings. Our partner...

Lanthanide-containing microgels as reagents for bioassays employing mass cytometry

2010/11-2014/15 • $178,215 • PI
This proposal describes research on the synthesis, characterization, and biofunctionalization of novel polymermicrogel beads with diameters on the order of 1 urn and encoded with different lanthanide metal ions. Theseinorganic-polymer hybrid microgels will be developed as potential reagents for...

Ultrasensitive multiplexed mass cytometry immunoassays based on single patch nanoparticles

2010/11-2012/13 • $391,800 • PI,CO
This proposal describes research on the synthesis of inorganic nanoparticles (NPs), particularly lanthanide-containing nanoparticles, and their conjugation to monoclonal antibodies. The NP-antibody complexes are designed for quantitative cellular immunoassays of biomarkers and transcripts of low...

Designer Peptides and Proteins: Characterization by High Pressure Liquid Chromatography

2012/13 • $70,036 • PI,CO
Key to chemical biology is purity of materials, whether for the design of new peptide/protein-modified hydrogels, drug colloids, or metal-chelating polymers. The high pressure liquid chromatograph (HPLC) allows both biomolecules to be purified and polymerization reaction kinetics to be...

Polymer coformation, dynamics, morphology and self-assembly

2010/11-2011/12 • $340,860 • PI
No summary

The design and processing of organized hybrid nanostructures

2010/11 • $199,600 • PI,CO
Over recent years there have been numerous discoveries in the area, of materials fabrication on the nanoscale. Researchers now believe that inspiring functionality can be built into systems made by organizing many molecular building blocks into one unit. The most advanced properties of such...

A preparative HPLC system for a multidisciplinary team of researchers

2010/11 • $75,821 • PI,CO
Three research programs (Yudin, Taylor, and Winnik) are in desperate need of a preparative HPLC system in order to cope with the challenging problems of separating highly polar peptide- and oligosaccharide molecules. The proposed unit will be located in the Yudin laboratory. The Taylor and...

Patents

FUSED-REFERENCE PARTICLE BASED NORMALISATION FOR IMAGING MASS SPECTROMETRY

This disclosure relates to reagents and their use for elemental imaging mass spectrometry of biological samples.
Related applications: EP20190858794, WO2019US50203, CA20193112016

ADDITIONAL MASS TAG POLYMERS FOR MASS CYTOMETRY

A new class of mass-tag polymers is provided, which include enriched metal isotopes such as zirconium and hafnium mass tags. The chemistry of these new mass tags are different from that of lanthanide mass tags, and opens up new mass channels that can be used in mass cytometry. These polymers may be used for mass cytometry, therapeutic delivery of a radioactive isotope, or screening of a therapeutic isotope. Aspects include a kit, method of making, and method of using a polymer, isotopic...
Related applications: EP20200849381, CA20203145861

METAL-LABELED POLYMER MICROBEADS WITH CONTROL OVER LABELING LEVEL

The present disclosure relates to metal labelled polymer microbeads and in particular to lanthanide labelled polymer microbeads for mass cytometry bead based assays and multiplexing applications. The polymer microbead comprises a copolymer comprising a structural monomer and a metal-chelating monomer.
Related applications: WO2022CA50386