Justin Powlowski

Key Research Areas
Associated Researchers
Associated Organizations

Recent Research Projects

NSERC Industrial Biocatalysis Network

2014/15-2018/19 • $5,150,000 • PI,CO
Oil and gas are converted to plastics, chemicals, and other industrial and consumer products through energy-intensive manufacturing processes. These products would be more environmentally sustainable if they were made from renewable resources, such as agricultural and forestry waste, and if they...

Pathways and Enzymes from Fungal Genomes for Degradation of Aromatic Compounds

2014/15-2018/19 • $175,000 • PI
In recent years there has been considerable interest in many countries, especially the US, in sequencing the genomes of fungi. Sequencing a genome nowadays is relatively easy and inexpensive, and there are over 150 fungal genome sequences available in public databases, with the number growing...

Discovery, characterization and applications testing of fungal enzymes for white biotechnology

2010/11-2015/16 • $302,100 • PI,CO
Being the most abundant global source of renewable biomass, plant derived lignocellulose is an attractive feedstock for the production of biofuels and chemicals. Enzymatic hydrolysis of pretreated feedstocks is presently the most effective method for converting the large polymers present in...

NSERC Bioconversion Network

2010/11-2013/14 • $4,363,700 • PI,CO
No summary

Enzymology of aromatic degradation by pseudomonas cf600 and selected fungi

2010/11-2011/12 • $90,000 • PI
Decomposition of organic matter in the biosphere is the task of all organisms on the Earth, but ultimately degradation of the nastiest and most complex molecules falls to bacteria and fungi. While these organisms have evolved to deal with a wide variety of natural substrates, they are...

Patents

CELL WALL DECONSTRUCTION ENZYMES OF AUREOBASIDIUM PULLULANS AND USES THEREOF

The present invention relates to process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a polypeptide having a.a polypeptide sequence as set forth in any one of SEQ ID Nos:3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 44, 47 and 50; b.a polypeptide that is at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%,98% or 99% homologous to the any one of SEQ ID Nos:3, 6, 9, 12, 15,...
Related applications: WO2013EP61769

NOVEL CELL WALL DECONSTRUCTION ENZYMES OF MYRIOCOCCUM THERMOPHILUM AND USES THEREOF

The present invention relates to a process for degrading biomass or pretreated biomass wherein an enzyme is used comprising a polypeptide having a. a polypeptide sequence as set forth in any one of SEQ ID Nos:3, 6, 9, 12, 15, 8, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 59, 61, 63, 65, 67, 69 and 71; b. a polypeptide that is at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%, 98% or 99% homologous to the any one of...
Related applications: WO2013EP61767

NOVEL CELL WALL DECONSTRUCTION ENZYMES OF SCYTALIDIUM THERMOPHILUM AND USES THEREOF

The present invention relates to process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a polypeptide having a. a polypeptide sequence as set forth in any one of SEQ ID Nos:3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 62 and 64; b. a polypeptide that is at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%, 98% or 99% homologous to the any one of SEQ ID...
Related applications: WO2013EP61768

CELL WALL DECONSTRUCTION ENZYMES OF PSEUDOCERCOSPORELLA HERPOTRICHOIDES AND|USES THEREOF

The present invention relates to novel Pseudocercosporella herpotrichoides enzymes or proteins for cell wall deconstruction, polynucleotide sequences encoding the polypeptides according to the invention, a production process for the enzymes according to the invention and the use of the enzymes according to the invention in various industrial processes.
Related applications: WO2013EP71463

CELL WALL DECONSTRUCTION ENZYMES OF THERMOASCUS AURANTIACUS AND USES THEREOF

The present invention relates tonovel Thermoascus aurantiacus enzymes or proteins for cell wall deconstruction, polynucleotide sequences encoding the polypeptides according to the invention, a production process for the enzymes according to the invention and the use of the enzymes according to the invention in various industrial processes.
Related applications: WO2013EP71466

CELL WALL DECONSTRUCTION ENZYMES OF MYCELIOPHTHORA FERGUSII (CORYNASCUS THERMOPHILUS) AND USES THEREOF

The present invention relates to novel Myceliophthora fergusii (Corynascus thermophilus) enzymes or proteins for cell wall deconstruction, polynucleotide sequences encoding the polypeptides according to the invention, a production process for the enzymes according to the invention and the use of the enzymes according to the invention in various industrial processes.
Related applications: WO2013EP71465

NOVEL CELL WALL DECONSTRUCTION ENZYMES OF SCYTALIDIUM THERMOPHILUM, MYRIOCOCCUM THERMOPHILUM, AND AUREOBASIDIUM PULLULANS, AND USES THEREOF

The present invention relates to novel polypeptides and enzymes (e.g., thermostable proteins and enzymes) having activities relating to biomass processing and/or degradation (e.g., cell wall deconstruction), as well as polynucleotides, vectors, cells, compositions and tools relating to same, or functional variants thereof. More particularly, the present invention relates to secreted enzymes that may be isolated from the fungi, Scytalidium thermophilum strain CBS 625.91, Myriococcum...
Related applications: WO2013CA50434, EP20130800985

CELL WALL DECONSTRUCTION ENZYMES OF PAECILOMYCES BYSSOCHLAMYDOIDES AND USES THEREOF

The present invention relates to a process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a.a polypeptide comprising an amino acid sequence of any one of SEQ ID NOs: or 6; b.a polypeptide comprising an amino acid sequence that is at least 85%, preferably at least 90%, more preferably at least 95%, even more preferably at least 96%, 97%, 98% or 99%amino acid sequence identity to the polypeptide defined in (a); c.a polypeptide comprising an amino...
Related applications: WO2014EP54946

CELL WALL DECONSTRUCTION ENZYMES OF MALBRANCHEA CINNAMOMEA AND USES THEREOF

The present invention relates to a novel process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising: a. a polypeptide comprising an amino acid sequence of any one of SEQ ID NOs: 5 or 6; b. a polypeptide comprising an amino acid sequence that is at least 70%, preferably at least 80%, more preferably at least 90%, even more preferably at least 95%, 96%, 97%, 98% or 99% amino acid sequence identity to the polypeptide defined in (a); c. a polypeptide...
Related applications: WO2014EP54949

NOVEL CELL WALL DECONSTRUCTION ENZYMES OF THIELAVIA AUSTRALIENSIS AND USES THEREOF

The present invention relates to a novel process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a. a polypeptide comprising an amino acid sequence of any one of SEQ ID NOs: 7- 9; b. a polypeptide comprising an amino acid sequence that is at least 85%, preferably at least 90%, more preferably at least 95%, even more preferably at least 96%, 97%, 98% or 99% amino acid sequence identity to the polypeptide defined in (a); c. a polypeptide comprising an...
Related applications: WO2014EP54954

NOVEL CELL WALL DECONSTRUCTION ENZYMES OF THERMOASCUS AURANTIACUS, MYCELIOPHTHORA FERGUSII (CORYNASCUS THERMOPHILUS), AND PSEUDOCERCOSPORELLA HERPOTRICHOIDES, AND USES THEREOF

The present invention relates to novel polypeptides and enzymes (e.g., thermostable proteins and enzymes) having activities relating to biomass processing and/or degradation (e.g., cell wall deconstruction), as well as polynucleotides, vectors, cells, compositions and tools relating to same, or functional variants thereof. More particularly, the present invention relates to secreted enzymes that may be isolated from the fungi, Thermoascus aurantiacus strain CBS 181.67, Myceliophthora...
Related applications: WO2013CA50778

NOVEL CELL WALL DECONSTRUCTION ENZYMES OF AMORPHOTHECA RESINAE, RHIZOMUCOR PUSILLUS, AND CALCARISPORIELLA THERMOPHILA, AND USES THEREOF

The present invention relates to novel polypeptides and enzymes (e.g., thermostable proteins and enzymes) having activities relating to biomass processing and/or degradation (e.g., cell wall deconstruction), as well as polynucleotides, vectors, cells, compositions and tools relating to same, or functional variants thereof. More particularly, the present invention relates to secreted enzymes that may be isolated from the fungi, Amorphotheca resinae strain DAOM194228, Rhizomucor pusillus...
Related applications: WO2014CA50024

NOVEL CELL WALL DECONSTRUCTION ENZYMES OF MALBRANCHEA CINNAMOMEA, THIELAVIA AUSTRALIENSIS, AND PAECILOMYCES BYSSOCHLAMYDOIDES, AND USES THEREOF

The present invention relates to novel polypeptides and enzymes (e.g., thermostable proteins and enzymes) having activities relating to biomass processing and/or degradation (e.g., cell wall deconstruction), as well as polynucleotides, vectors, cells, compositions and tools relating to same, or functional variants thereof. More particularly, the present invention relates to secreted enzymes that may be isolated from the fungi, Malbranchea cinnamomea strain CBS 343.55, Thielavia australiensis...
Related applications: WO2014CA50228

NOVEL CELL WALL DECONSTRUCTION ENZYMES OF CHAETOMIUM THERMOPHILUM, THERMOMYCES STELLATUS, AND CORYNASCUS SEPEDONIUM, AND USES THEREOF

The present invention relates to novel polypeptides and enzymes (e.g., thermostable proteins and enzymes) having activities relating to biomass processing and/or degradation (e.g., cell wall deconstruction), as well as polynucleotides, vectors, cells, compositions and tools relating to same, or functional variants thereof. More particularly, the present invention relates to secreted enzymes that may be isolated from the fungi Chaetomium thermophilum strain ATCC 16451, Thermomyces stellatus...
Related applications: WO2015CA50042

NOVEL CELL WALL DECONSTRUCTION ENZYMES OF REMERSONIA THERMOPHILA (STILBELLA THERMOPHILA), MELANOCARPUS ALBOMYCES, AND LENTINULA EDODES, AND USES THEREOF

The present invention relates to novel polypeptides and enzymes (e.g., thermostable proteins and enzymes) having activities relating to biomass processing and/or degradation (e.g., cell wall deconstruction), as well as polynucleotides, vectors, cells, compositions and tools relating to same, or functional variants thereof. More particularly, the present invention relates to secreted enzymes that may be isolated from the fungi Remersonia thermophila (Stilbella thermophila) strain ATCC 22073,...
Related applications: WO2015CA51283

NOVEL CELL WALL DECONSTRUCTION ENZYMES OF RHIZOMUCOR MIEHEI, THERMOASCUS THERMOPHILUS (DACTYLOMYCES THERMOPHILUS), AND HUMICOLA HYALO THERMOPHILA, AND USES THEREOF

The present invention relates to novel polypeptides and enzymes (e.g., thermostable proteins and enzymes) having activities relating to biomass processing and/or degradation (e.g., cell wall deconstruction), as well as polynucleotides, vectors, cells, compositions and tools relating to same, or functional variants thereof. More particularly, the present invention relates to secreted enzymes that may be isolated from the fungi Rhizomucor miehei strain CBS 182.67, Thermoascus thermophilus...
Related applications: WO2015CA51284

NOVEL CELL WALL DECONSTRUCTION ENZYMES OF CHAETOMIUM OLIVICOLOR, ACREMONIUM THERMOPHILUM, AND MYCELIOPHTHORA HINNULEA, AND USES THEREOF

The present invention relates to novel polypeptides and enzymes (e.g., thermostable proteins and enzymes) having activities relating to biomass processing and/or degradation (e.g., cell wall deconstruction), as well as polynucleotides, vectors, cells, compositions and tools relating to same, or functional variants thereof. More particularly, the present invention relates to secreted enzymes that may be isolated from the fungi Chaetomium olivicolor strain CBS 102434, Acremonium thermophilum...
Related applications: WO2015CA51285