Dominique Michaud

Key Research Areas
Associated Researchers
Associated Organizations

Recent Research Projects

AgroPhytoSciences, un programme de formation FONCER du CRSNG pour relever les défis technologiques environnementaux et socioéconomiques de la production végétale

2014/15-2018/19 • $1,340,000 • PI,CO
Le programme AgroPhytoSciences se fonde sur le principe que les scientifiques de la prochaine génération doivent être aptes à travailler dans un contexte pluridisciplinaire et qu'ils doivent acquérir l'entrepreneuriat nécessaire au développement de nouveaux produits. Le programme...

Environmental factors controlling the production of recombinant proteins in Nicotiana benthamiana plants grown hydroponically in mobile gullies

2017/18-2018/19 • $611,836 • PI,CO
The overall goal of this project is to determine the main factors regulating transient expression and accumulation of recombinant vaccine antigens and other biopharmaceuticals in Nicotiana benthamiana (NB) cultivated hydroponically in moving gullies. This type of cultivation system offers great...

Hormone-medicated improvement of recombinant protein yields in plants

2016/17-2018/19 • $240,440 • PI
The use of plants as bio-factories for the production of clinically useful recombinant proteins has been bolstered in recent years by the approval of a first plant-made biopharmaceutical for human therapy, the demonstrated feasibility of readily producing therapeutic antibodies in plants during...

Recombinant cystatins in plants

2015/16-2018/19 • $228,000 • PI
Protease inhibitors have long been recognized as useful biopesticides for the development of pest-resistant transgenic crops. Recent studies have also described indirect effects for these proteins on the modified plant’s own physiology, potentially useful to introduce such new traits of...

Physiological determinants of recombinant protein expression in nicotiana benthamiana

2013/14-2016/17 • $1,091,478 • PI,CO
This project will study and optimize the physiological determinants of vaccine antigen production in the Nicotiana benthamiana (Nb) plant protein expression platform, in relation with a number of cultural and growth parameters including light regime, atmospheric CO2, nitrogen fertilization and...

Recombinant cystatins in plants

2010/11-2014/15 • $250,000 • PI
Several studies documented the usefulness of recombinant protease inhibitors for the development of insect-resistant transgenic crops, but a number of recent studies also described unintended effects of these proteins modifying the host plant's metabolic characteristics. Based on novel...

New generation microplate reader

2013/14 • $22,507 • PI,CO
Dominique Michaud and Charles Goulet, from the Département de phytologie of Laval University in Québec City, are seeking financial support from NSERC Research Tools and Instruments Grants Program for the acquisition of a SpectroMax Plus 384 microplate reader. Drs. Michaud and Goulet lead...

Recombinant cystatins in plants

2010/11-2012/13 • $120,000 • PI
No summary

Companion protease inhibitors for the protection of clinically-useful recombinant proteins in plants

2010/11 • $124,172 • PI,CO
Several proteins of medical interest have been produced successfully in « plant protein biofactories » over the last ten years, including a variety of vaccines, diagnostic antibodies, protein allergens and peptidic hormones. The main goal of this project is to develop a plant expression system...



A method for synthesizing a protein of interest within a plant or a portion of a plant is provided. The method involves treating the plant to increase secondary leaf biomass production, followed by introducing one or more than one nucleic acid sequence encoding a protein of interest operatively linked with a regulatory region that is active in the plant, into the plant. The plant is then maintained under conditions that permit the nucleic acid sequence encoding the protein of interest to be...
Related applications: CA20152954759, WO2015CA50644, ES20150819391T, EP20150819391


A method of increasing expression of an heterologous protein of interest in a plant or portion of the plant is provided. The method comprises treating the plant or portion of the plant with ajasmonate-pathway activator, and introducing a nucleotide sequence operably linked to a regulatory region derived from a DNA plant virus and encoding the heterologous protein of interest into the plant or portion of the plant. Alternatively, the plant or plant portion may comprise the nucleic acid and...
Related applications: EP20160816892, CA20162991139, WO2016CA50772