Recent Research Projects

Single-molecule manipulation and imaging platform

2018/19-2019/20 • $350,000 • PI
Macroscopic properties of materials and reagents are governed by molecular-scale interactions. In some fields, subtle details of these interactions can completely change the property of a compound or material. For example, can a new cancer drug be engineered to bind only to the target cell...

Single-molecule visualization and manipulation of protein and DNA interactions and dynamics, on nanoscale dimensions

2017/18-2019/20 • $138,000 • PI
The overarching objective of my research program is to visualize, quantitatively analyze and understand protein-DNA and protein-protein interactions, which play key biophysical roles in regulating cellular processes. I am fascinated by the interplay between the dynamic, structural, and genetic...

Identify and quantitate biomarkers using nanopores fabricated by controlled breakdown

2016/17 • $242,940 • PI,CO
As nanometer-scale holes embedded in thin insulating membranes, nanopores provide a confined space withinwhich single molecules can be captured and electrically interrogated with high throughput. However commercialization of nanopore-based technologies for health science applications, including...

Massively parallel linearization and chemical treatment of DNA molecules using tunable nanoscale confinement

2015/16-2016/17 • $241,330 • PI
Genetic analysis, such as DNA sequencing, has become an established technology for health science research, as well as disease prognosis and diagnosis. However, modern sequencing methods face considerable challenges, presenting key technology development opportunities. For instance, BCC Research...

Single-molecule investigations of fast biomolecular search processes initiating DNA repair

2012/13-2016/17 • $125,000 • PI
The overarching objective of my research program is to create a new mechanistic understanding of the fast molecular search processes, which underlie a host of key cellular processes. One class of fast molecular search processes, that remain largely uncharacterized due to limited analysis...

Nanoscale toolset for formatiing and visualizing single molecules

2016/17 • $125,000 • PI
Dr. Sabrina Leslie's Laboratory has developed a unique system for formatting and visualizing molecules withinnanoscale surface-embedded topographies (nanoSET), which are fabricated in thin glass coverslips. Byconfining macromolecules and soft materials, such as protein and DNA, within...

Market assessment of "molecular manipulation and nanoscopy platform"

2015/16 • $9,000 • PI
Professor Leslie has developed a novel "Molecular Manipulation and Nanoscopy Platform" which enables single-molecule visualization of a wide range of molecular processes, with applications to materials science, chemistry, nanotechnology, medicine, and biology. Recently, her research and...

Nanoconfinement based single-cell cytogenetic platform for pre-/post-implantation genetic diagnosis

2013/14-2014/15 • $219,185 • PI,CO
Chromosomal analysis is routinely performed in Canada when there is an elevated risk of chromosomal anomalies (e.g. inthe prenatal period due to maternal age, family history of a chromosomal rearrangement). In particular, pre-implantationgenetic diagnosis (PGD) is performed on 8-cell stage...

Visualizing and Directing Dynamic DNA Nanostructure Assembly With Single-Molecule Resolution

2014/15 • $150,000 • PI,CO
The use of DNA in nanotechnology has become increasingly widespread with far-reaching advantages: these natural nanoscale building blocks are self-assembling, are able to organize species such as drugs, nanoparticles, and other biomolecules, and can store and read information to target specific...

Single-DNA manipulation above a suspended membrane using tunable nanoscale confinement

2014/15 • $25,000 • PI
Abbott's mandate is to discover new technologies to improve the performance and cost of clinical diagnosticassays. Recently, Abbott has invested in single-molecule detection as part of developing new clinical in vitrodiagnostics (IVD). Critically, Abbott faces the challenge of controlling the...

Imaging Patterned Materials Under Strain

2014/15 • $25,000 • PI
to withstand stress is a primary limiting factor in determining component lifetime and usability. Rolls Royce'spartnership with McGill Physics will perform new research to develop, understand and optically characterizenew kinds of materials, ultimately for use in turbine engine components with...

A custom inexpensive versatile microscope

2013/14 • $25,000 • PI
The study of muscle contraction is important to understand most functions of life, including locomotion, heartbeating and lung mechanics. While the mechanisms of contraction lie in sub-cellular structures, their study hasbeen challenging as the necessary technology is not readily available. The...

Dramatically improved single-molecule imaging of thin molecular films

2012/13 • $25,000 • PI
Quorum Technologies seeks to develop imaging devices capable of probing a wider range of molecular interactions than standard technologies allow, with single-molecule resolution. Existing single-molecule microscopy techniques are inadequate for probing the physical mechanisms of many important...

Force-and-fluorescence microscopy of DNA repair

2011/12 • $147,686 • PI
Microscopically, the process of DNA repair is initiated by an extended search performed by protein molecules over complex DNA substrates. Characterizing the mechanisms by which molecules interrogate and interpret the topological features of a DNA substrate, and ultimately bind to break sites, is...

Patents

MINIATURIZED FLOW CELL AND SYSTEM FOR SINGLE-MOLECULE NANOCONFINEMENT AND IMAGING

Nanofluidic flow cells and systems for single-molecule nanoconfinement and imaging of molecules in a fluid are described. The nanofluidic flow cell comprises a bottom substrate bonded to a top substrate, microchannels and a central chamber carved in the bottom or top substrate. The microchannels and the central chamber define an empty space into which a fluid can flow. The microchannels extend on opposite side of the central chamber, each microchannel comprising a central portion crossing...
Related applications: WO2018IB58023