CANCER DETECTION, CLASSIFICATION, PROGNOSTICATION, THERAPY PREDICTION AND THERAPY MONITORING USING METHYLOME ANALYSIS

Inventors
  • BRATMAN, Scott,
  • BURGENER, Justin,
  • CHAKRAVARTHY, Ankur,
  • DE CARVALHO, Daniel
  • SHEN, Shu Yi
  • SINGHANIA, Rajat,
Documents
Applicants
  • Sinai Health System
  • University Health Network
Contact Info
Mark Taylor
Director, Technology Development & Commercialization
(416) 581-7404
[email protected]
Michael Hanna
Sr. Business Development Officer
(416) 586-4800 ext. 4752
[email protected]

Abstract

There is described herein a method of detecting the presence of DNA from cancer cells in a subject comprising: providing a sample of cell-free DNA from a subject; subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; optionally denaturing the sample; capturing cell-free methylated DNA using a binder selective for methylated polynucleotides; sequencing the captured cell-free methylated DNA; comparing the sequences of the captured cell- free methylated DNA to control cell-free methylated DNAs sequences from healthy and cancerous individuals; identifying the presence of DNA from cancer cells if there is a statistically significant similarity between one or more sequences of the captured cell- free methylated DNA and cell-free methylated DNAs sequences from cancerous individuals.