CANCER DETECTION, CLASSIFICATION, PROGNOSTICATION, THERAPY PREDICTION AND THERAPY MONITORING USING METHYLOME ANALYSIS

Contacts

Contact University Health Network


Contact Sinai Health System

Inventors
  • DE CARVALHO, Daniel
  • BRATMAN, Scott
  • CHAKRAVARTHY, Ankur
  • SINGHANIA, Rajat
  • BURGENER, Justin
  • SHEN, Shu Yi
Documents
Applicants
  • University Health Network
  • Sinai Health System

Abstract

There is described herein a method of detecting the presence of DNA from cancer cells in a subject comprising: providing a sample of cell-free DNA from a subject; subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; optionally denaturing the sample; capturing cell-free methylated DNA using a binder selective for methylated polynucleotides; sequencing the captured cell-free methylated DNA; comparing the sequences of the captured cell- free methylated DNA to control cell-free methylated DNAs sequences from healthy and cancerous individuals; identifying the presence of DNA from cancer cells if there is a statistically significant similarity between one or more sequences of the captured cell- free methylated DNA and cell-free methylated DNAs sequences from cancerous individuals.